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The Journal of Internal Korean Medicine 2012;33(2): 197-208. |
| 皂角刺에서 PC-3 생장 억제 성분 정제 |
| 임세현1, 이병호2, 김영균3, 조수인4, 김용성5, 임지연6 |
1극동대학교 간호학과
2홍재한의원
3동의대학교 한의학과
4부산대학교 한의학과
5동신대학교 한의학과
6동국대학교 의학과 |
| Purification of Anti PC-3 Prostate Cancer Agents from Gleditsiae Spina |
| Se-hyun Lim1, Byung-ho Lee2, Young-gyun Kim3, Su-in Cho4, Yong-seong Kim5, Chi-yeon Lim6 |
1Dept. of Nursing, Far East University
2Hong Jae Korean Medical Clinic
3Dept. of Korean Medicine, Dong-Eui University
4Dept. of Korean Medicine, Pu-San National University
5Dept. of Korean Medicine, Dong-Shin University
6Dept. of Medicine, Dong-Guk University |
| Correspondence |
Chi-yeon Lim ,Tel: 031-961-8431, Fax: 031-961-8431, Email: rachun@hanmail.net
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Published online: June 30, 2012. |
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| ABSTRACT |
Objectives:
Gleditsiae Spina has the effects of expelling toxins, draining pus, invigorating blood and resolving abscesses. Some clinicians apply the herb for patients suffering from cancer. However, its anti-cancer activities are not well understood. In the present study, anti-tumor agents from Gleditsiae Spina were purified.
Methods :
The viability of the PC-3 cell line was determined using MTT assay, and the induction of apoptosis by Gleditsiae Spina extract in PC-3 cells was measured by Annexin-V/propidium iodide double staining assay detected by flow cytometry. TLC and HPLC analysis were used to separate and identify the anti-cancer agents.
Results:
Treatment of the extract resulted in significant decreased cell viability of PC-3 cells in a dose- and time-dependent manner. Dose-dependent apoptotic cell death was also measured by flow cytometry analysis. The anti-cancer agents were successfully separated and identified by using TLC and HPLC analysis and the most potential agent among them was separated from EtOAC fraction.
Conclusions:
These results might be applied in developing new drugs from natural resources like Korean traditional medicine, and also support the clinical usefulness of herbal medicine. |
| Key words:
Gleditsiae Spina, PC-3, apoptosis |
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