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The Journal of Internal Korean Medicine 2009;30(1): 24-35. |
| 牧丹皮가 손상된 성상신경세포의 CD81 및 GFAP의 발현에 미치는 영향 |
| 문성진1, 성기문1, 임진영2, 송봉근1 |
1원광대학교 한의과대학 내과학교실
2원광대학교광주병원 재활의학과 |
| The Effect of the Moutan Radicis Cortex on Expression of CD81 and GFAP
in Injured Astrocyte |
| Sung-jin Moon1, Ki-moon Seon1, Jin-young Lim2, Bong-keun Song1 |
1Dept. of Internal Medicine, Wonk-wang University Oriental Medical School
2Dept. of Rehabilitation, Wonk-wang University Medical Center |
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Published online: March 30, 2009. |
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| ABSTRACT |
Object:
In conditions of brain infarction, irreversible axon damage occurs in the central nerve system (CNS), because gliosis makes physical and mechanical barriers. If gliosis formation could be suppressed, irreversible axon damage would be reduced. This could mean that an injured CNS could be regenerated. CD81 and GFAP have close relationships to gliosis. The increase in glial cells at CNS injury gives rise to the expression of CD81 and GFAP. CD81 was postulated to play a central role in the process of CNS scar formation.
Method:
In this study, the author investigated the effect of the water extract of the Moutan Radicis Cortex on regulation of CD81 and GFAP expression in injured CNS cells. MTT assay was used to examine cell viability, while RT-PCR and ELISA methods were carried out to measure the expression of CD81 and GFAP in the astrocyte.
Results:
We observed that water extract of the Moutan Radicis Cortex increased cell viability under hypoxia induced by CoCl2 and suppressed the expression of CD81 and GFAP up-regulated by hypoxia.
Conclusion:
These results suggest that the Moutan Radicis Cortex could promote neural regeneration as a consequence of protecting CNS cells from hypoxia and suppressing the reactive gliosis following CNS injury |
| Key words:
gliosis, CD81, GFAP, CNS injury, hypoxia, Moutan Radicis Cortex |
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