A Comparison Study on Effects of <i>>Jisildoche-hwan</i> Extract and Misoprostol on
Indomethacin-induced Gastric Mucosal Lesions in Mice

Lee, Seul-hee; Baek, Tae-hyun

The Journal of Internal Korean Medicine > Volume 29(4); 2008 > Article

Original Article

The Journal of Internal Korean Medicine 2008;29(4): 939-949.

Indomethacin으로 유발된 생쥐의 위점막 손상에 대한 枳實導滯丸과 Misoprostol의 효과 비교 연구

이슬희, 백태현

상지대학교 부속 한방병원 내과

A Comparison Study on Effects of >Jisildoche-hwan Extract and Misoprostol on Indomethacin-induced Gastric Mucosal Lesions in Mice

Seul-hee Lee, Tae-hyun Baek

Dept. of internal Medicine Oriental Hospital of Sangji University

Correspondence

Seul-hee Lee ,Tel: 033-741-9382, Fax: 033-743-7184, Email: 3-honey@hanmail.net

Published online: December 30, 2008.

ABSTRACT

Objective :
This study was carried out to investigate the effects of Jisildochehwan extract on indomethacin-induced gastric mucosal lesions of mice, as compared with misoprostol.

Methods :
Experimental mice were classified into four groups. No gastro-inflammation elicited mice were the normal group(NOR). Gastro-inflammation elicited mice were the control group(CON). Misoprostol-administered mice after gastro-inflammation elicitation were the misoprostol-administered group(MA). Jisildochehwan-administered mice after gastro-inflammation elicitation were the Jisildochehwan-administered group(JA). In this study, we examined superoxide dismutase(SOD) ability, anti-inflammatory ability arrangement of mucous-secreted cells, distribution of mucosal neck cells, mucosal surface cells, neutral mucous-secreted cells, PAS reaction, COX-1, TNF-α, NF-κB p65 and iNOS.

Results:
The SOD ability of the Jisildochehwan group increased dose-dependently. The hemorrhagic erosion and the damage of arrangement of mucous-secreted cells increased in the CON group, but decreased in the MA and JA groups. The COX-1 positive were decreased in the CON group, but increased in the MA and JA groups. The distribution of mucosal neck cells and mucosal surface cells, PAS positive reaction of surface mucous cells were decreased in CON group, but increased in MA and JA group. TNF-α, NF-κB p65 and iNOS increased in the CON group, but decreased in the MA and JA groups. In all the results, the effects were better in the JA group than in the MA group.

Conclusion:
Jisildochehwan extract was more effective than Misoprostol. Jisildochehwan has excellent protective effects on indomethacin-induced gastric mucosal lesions.

Key words: Jisildoche-hwan extract, indomethacin, Misoprostol, Gastric mucosa