| 부자의 메탄올 추출물에 의한 신세포암의 증식억제 및 세포사멸 유도 |
| 김종환1, 최영현2, 황원덕1 |
1동의대학교 한의과대학 신계내과학교실
2동의대학교 한의과대학 생화학교실 |
| Inhibition of Cell Proliferation and Induction of Apoptosis by Methanolic Extract of
Aconiti Tuber in Human Renal Cell Carcinoma Cells |
| Jong-hwan Kim1, Yung-hyun Choi2, Won-deuk Hwang1 |
1Department of Oriental Medicine, Dongeui University
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| Correspondence |
Won-deuk Hwang ,Tel: 051-850-8625, Fax: 051-867-5162, Email: hwooso@hanafos.com
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Published online: March 30, 2008. |
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| ABSTRACT |
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Aconiti Tuber is a traditional medicinal plant generally used in Oriental medicine therapy. In this study, we investigated the biochemical mechanisms of anti-proliferative effects by the methanol extract of Aconiti tuber (MEBJ) in Caki-1 human renal cell carcinoma cells. It was found that MEBJ could inhibit, in a dose-dependent manner, cell growth which was associated with apoptotic cell death such as formation of apoptotic bodies, DNA fragmentation and increased populations of apoptotic-sub G1 phase. Apoptosis of Caki-1 cells by MEBJ was associated with an up-regulation of pro-apoptotic Bax expression, and a down-regulation of anti-apoptotic Bcl-2 in a dose-dependent manner; however, the levels of IAP family were not affected. MEBJ treatment also induced the proteolytic activation of caspase-3 and -8, and a inhibition of poly(ADP-ribose) polymerase (PARP) and PLCγ1 protein. Furthermore, MEBJ treatment caused a dose-dependent inhibition of iNOS and cyclooxygenase-2 (Cox-2). Though further studies will be needed to identify the active compounds that confer the anti-cancer activity of MEBJ, the present findings provide important new insights into the possible molecular mechanisms of the apoptotic activity of MEBJ in cancer cells. |
| Key words:
Aconiti tuber, Renal cell carcinoma, Apoptosis, Bax protein, Death receptors. |
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