| 麻黃潤肺湯이 호흡기 뮤신 분비 및 기관지 평활근에 미치는 영향 |
| 황지호, 양수영, 변준섭, 박양춘 |
| 대전대학교부속한방병원 폐계내과학교실 |
| Effect of Mahwangyunpye-tang on Secretion of Airway Mucin and Tracheal Smooth Muscle |
| Ji-ho Hwang, Su-young Yang, Jun-seop Byun, Yang-chun Park |
| Division of Respiratory system, Dept. of Internal Medicine, College of Oriental Me야cine, Daejeon University |
| Correspondence |
Yang-chun Park ,Tel: 043-229-3704, Fax: 043-253-8757, Email: omdpyc@dju.ac.kr
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Published online: December 30, 2007. |
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| ABSTRACT |
Objectives:
The purpose of this study was to investigate whether Mahwangyunpye-tang(MYT) significantly affects mucin secretion from airway epithelial cells.
Methods :
Confluent hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of MYT to assess the effect of the agent on 3H-mucin secretion. Total elution profiles of control spent media and treatment sample through Sepharose CL-4B column were analyzed. Effect of MYT on contractility of isolated tracheal smooth muscle was investigated; also investigated was effect of the agent on MUC5AC gene expression in cultured NCI-H292 cells. Possible cytotoxicities of the agent were assessed both by measuring lactate dehydrogenase (LDH) release from HTSE cells and examining the rate of survival and proliferation of NCI-H292 cells.
Results:
MYT significantly increased mucin secretion from cultured HTSE cells, without significant cytotoxicity. MYT chiefly affected the 'mucin' secretion. MYT inhibited Acetylcholine-induced contraction of isolated tracheal smooth muscle. MYT did not significantly affect the expression levels of MUC 5AC gene in cultured NCI-H292 cells.
Conclusions:
Based on the above results, it is suggested that MYT increased mucin secretion from cultured HTSE cells without significant cytotoxicity and inhibited contraction of isolated tracheal smooth muscle. |
| Key words:
airway, mucin, Mahwangyunpye-tang (MYT), MUC5AC mRNA |
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