Peroxynitrite Scavenging Activity of <i>Samjunghwan</i>

Lee, Dong-joon; Jeong, Ji-cheon

The Journal of Internal Korean Medicine > Volume 27(1); 2006 > Article

Original Article

The Journal of Internal Korean Medicine 2006;27(1): 178-187.

三精丸의 Peroxynitrite 제거 효과

이동준, 정지천

동국대학교 한의과대학 내과학교실

Peroxynitrite Scavenging Activity of Samjunghwan

Dong-joon Lee, Ji-cheon Jeong

Dept. of Internal Medicine, College of Oriental Medicine, Dongguk University

Correspondence

Ji-cheon Jeong ,Tel: 054-770-1265, Fax: 054-770-1500, Email: jjcjh@paran.com

Received: February 14, 2006, Accepted: February 27, 2006, Published online: March 30, 2006.

ABSTRACT

Objectives:
Peroxynitrite (ONOO^-), ․O2 ^- and nitric oxide (NO) are cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer’s disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate ONOO^- scavenging activities, and that of its precursors, NO and ․O2 ^- of Samjunghwan.

Methods :
To investigate ONOO^-, NO, ․O2 ^- scavenging activities, fluorescent probes, namely 2',7'-dichlorodihydrofluorescein diacetate (DCFDA), 4,5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used.

Results:
Samjunghwan markedly scavenged authentic ONOO^-, ․O2 ^- and NO. It also inhibited ONOO^- induced by ․O2 ^- and NO which are derived from SIN-1. Furthermore, Samjunghwan inhibited ONOO^-, ․O2 ^- and NO generation in LPS-treated ICR mouse kidney postmitochondria.

Conclusions:
These results suggest that Samjunghwan is an effective ONOO^-, ․O2 ^- and NO scavenger, and that this substance has a potential role as an inhibitor of aging process, and in therapy against age-related diseases.

Key words: Samjunghwan, peroxynitrite, nitric oxide, reactive oxygen species