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The Journal of Internal Korean Medicine 2006;27(1): 166-177. |
| 瓦松이 만성 골수성 백혈병 세포주(K562)에서 세포사멸에 미치는 영향 |
| 윤경수, 김영철, 이장훈, 우홍정 |
| 경희대학교 한의과대학 간계내과학교실 |
| Effect of Orostschys japonicus A. Berger on Apoptosis in K562 Cell Lines |
| Kyoung-su Yun, Young-chul Kim, Jang-hoon Lee, Hong-jung Woo |
| Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University |
| Correspondence |
Hong-jung Woo ,Tel: 02-958-9118, Fax: 02-958-9120, Email: hjwoo@khu.ac.kr
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Received: February 10, 2006, Accepted: February 27, 2006, Published online: March 30, 2006. |
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| ABSTRACT |
Objectives:
This study was performed to determine if Orostschys japonicus A. Berger has protective effects against CML in K562 cell lines.
Materials and Methods :
MTT assay, cell proliferation assay, Reverse transcription-polymerase reaction chain, RT-PCR, DNA fragmentation assay, Quantitative PCR were studied.
Results:
Orostschys japonicus A. Berger had no effects on Bax gene in K562 cell lines, but decreased Bcl-2 gene, and increased the Caspases-3 gene. This is indicative of induced apoptosis in K562 cell lines by Orostschys japonicus A. Berger.
Conclusion:
These results suggest that Orostschys japonicus A. Berger has effects on apatosis in K562 cell lines. |
| Key words:
Orostschys japonicus A. Berger, K562 cell lines, apoptosis, Bax, Bcl-2, Caspase |
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