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The Journal of Internal Korean Medicine 2005;26(4): 853-863. |
| 인진이 간성상세포의 섬유화 억제에 미치는 영향에 대한 연구 |
| 김영철, 이장훈, 우홍정 |
| 경희대학교 한의과대학 간계내과학교실 |
| Inhibitory Effect of Artemisiae Capillaris Herba on Fibrogenesis in Primary Cultured Rat Hepatic Stellate Cells |
| Young-chul Kim, Jang-hoon Lee, Hong-jung Woo |
| Dept. of Internal Medicine, College of Oriental Medicine, Kyunghee University |
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Received: September 5, 2005, Accepted: October 10, 2005, Published online: December 30, 2005. |
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| ABSTRACT |
Object:
This study was performed to investigate the anti-fibrogenic effect of Artemisiae Capillaris Herba(ACH) on cultured rat hepatic stellate cells.
Methods :
Hepatic Stellate Cells were obtained from a 350gm Sprague-Dawley rat by tissue perfusion system, and the cells for the study were selected after 3 passages of culture on non-coated plastic culture dishes which enable the cells to activate, thus producing collagens in the cell media. Cells were treated with various concentrations of Artemisia Capillaris Herba(ACH) extract powder for 24 or 48 hours. After the treatment, Soluble collagen, procollagen levels and the mRNA of the procollagen type I C were measured by using assay kit and RT-PCR method.
Results:
Procollagen production by the hepatic stellate cells decreased after the treatment in a time-dependent dose-dependent manner. The mRNA expression decreased consistently with the volume of the secreted procollagen which indicates the herb has inhibitory effect on fibrogenesis of the liver by regulating one of the fibrosis associated genes in transcription.
Conclusion:
These results suggest that ACH is beneficial in the treatment of cirrhotic patients as well as for the patients with chronic hepatitis. |
| Key words:
Hepatic Stellate Cells(HSCs), Artemisiae Capillaris Herba(ACH), Liver Cirrhosis, collagen, RT-PCR |
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