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The Journal of Internal Korean Medicine 2006;27(3): 725-736. |
| Human Neuroblastoma Cell Line BE2에 대한 健脾補腎抗癌湯의 세포고사 기전 연구 |
| 조영기1, 문미현1, 이성균1, 정현애2, 이정섭1, 남상규1, 문구1, 신선호1, 김동웅3 |
1원광대학교 한의과대학 전주한방병원
2원광대학교 한의과대학 익산한방병원
3서울 광동한방병원 |
| Study of The Apoptotic Mechanisms of Gunbibosinhangam-tang on Human Neuroblastoma Cell Line BE2 |
| Young-Kee Cho1, Mi-Hyun Moon1, Seong-Kyun Lee1, Hyun-Ae Jeong2, Jung-Sub Lee1, Sang-Kyu Nam1, Goo Moon1, Sun-Ho Shin1, Dong-Woung Kim3 |
1Wonkwang University Jeonju Oriental Medicine Hospital, Jeonju, Korea
2Wonkwang University Iksan Oriental Medicine Hospital, Iksan, Korea
3Kwangdong Oriental Medicine Hospital, Seoul, Korea |
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Published online: September 30, 2006. |
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| ABSTRACT |
Objective:
In order to investigate cell death mechanisms by Gunbibosinhangam-Tang(G.B.H) in cancer cells, the activities of apoptosis signaling pathway were tested in human neuroblastoma cell line BE2.
Methods:
Viability of BE2 cells was markedly decreased by treatment of the water extract of G.B.H in a dose-dependent manner. G.B.H-induced cell death was confirmed as apoptosis characterized by chromatin condensation. We tested whether the water extract of G.B.H affects the anti-apoptotic proteins such as Bcl-XL.
Results:
Bcl-XL was uneffected by the addition of the water extract of G.B.H. in a time-dependent manner. Cleavage of PARP(poly-ADP-ribose polymerase) by activation of caspase-8 protease was also observed in BE2 cells by the treatment of the water extract of G.B.H.
Conclusion:
These results suggest that the water extract of G.B.H exerts anti-cancer effects on human neuroblastoma BE2 cells by inducing the apoptotic death via activation of intrinsic caspase cascades. |
| Key words:
apoptosis, neuroblastoma, Gunbibosinhangam-Tang |
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