Interleukin-5 Inhibition Assay of the Oriental Materia Medica Treatment by High Through Screening on the Splenocyte of Mouse

Park, Dong-hee; Rhee, Hyung-koo; Jung, Sung-ki; Jung, Hee-jae

The Journal of Internal Korean Medicine > Volume 27(2); 2006 > Article

Original Article

The Journal of Internal Korean Medicine 2006;27(2): 394-406.

High through screening(HTS)를 이용한 白鼠의 脾臟細胞에서의 IL-5 분비 조절 韓藥物 Screening

박동희, 이형구, 정승기, 정희재

경희대학교 한의과대학 폐계내과학교실

Interleukin-5 Inhibition Assay of the Oriental Materia Medica Treatment by High Through Screening on the Splenocyte of Mouse

Dong-hee Park, Hyung-koo Rhee, Sung-ki Jung, Hee-jae Jung

Division of Allergy & Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyunghee University

Correspondence

Hee-jae Jung ,Tel: 02-958-9147, Fax: 02-958-9148, Email: hanfish@khmc.or.kr

Received: April 15, 2006, Accepted: June 15, 2006, Published online: June 30, 2006.

ABSTRACT

Backgrounds and Objectives:
Asthma is considered to be an inflammatory disease characterized by airway hyperresponsiveness and pulmonary eosinophilia. And it is known the structure and function of IL-5, its receptor and the mechnism IL-5 triggered eosinophil accumulation and inflammaion of the airways. At this point of view, we assume which oriental materia medica can the splenocyte inhibit from secreting the IL-5 in vitro. Material and

Methods:
We used the splenocyte of mouse 8 weeks after its birth, and then cultivated those into the 2 experimental groups and control group for 48 hours. The culture medium of experimental groups were made of 1㎍/㎖, 10㎍/㎖oriental materia medica, representative. And the culture media of control group was given no oriental materia medica. Then, we assayed the quantity of cytokine-expression by the Sandwich ELISA. The quantities of cytokine-expression of the experimental groups were compared with that of the control group which was standardized. These method were used for the all of oriental materia medica treated.

Results:
In this study, we demonstrated that 12 oriental materia medica that inhibit the splenocyte from secreting the IL-5 in both 1㎍/㎖ and 10㎍/㎖ culture media. Those were Equiseti Herba, Sophorae Subprostratae Radix, Moutan Radicis Cortex, Trichosanthis Radix, Buddleiae Flos, Cyperi Rhizoma, Benincasae Semen, Armeniacae Semen, Zedoariae Rhizoma, Astragali Semen, Dolichoris Semen, Lilii Bulbus, Asparagi Radix, Atractylodes Rhizoma White, Polygonati Officinallis Rhizoma.

Conclusions:
These findings indicate that some oriental materia medica, specially Antipyretics, Herbs for Resolving Phlegm, Relieving Cough and Calming Wheezing and Herbs for Tonifying and Invigorating effects inhibit the splenocyte from secreting the IL-5. And further study experimented in vivo is needed for treating IL-5-driven inflammatory disease including asthma.

Key words: Oriental materia medica, IL-5, asthma